Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
demonstrating growth in a nitrogen free medium
All of these
measuring15N2 by mass spectrometer

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Microbiology of Soils
A heterocyst is

a type of spore
the progenitor of cyanobacterial vegetative cells
a terminally differentiated cell that fixes nitrogen
a cell that carries out oxygenic photosynthesis

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Microbiology of Soils
The groups of symbiotic bacteria, which have the ability to fix nitrogen

derive their food and minerals from the legume, and in turn they supply the legume with some or all of its nitrogen
these bacteria are from the genus, Rhizobium
All of these
grow together for a mutual benefit is called symbiosis and so these bacteria are called symbiotic nitrogen-fixing bacteria

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