Carbohydrate
In lysozyme catalysis, which of the following does not contribute?

Formation of a covalent intermediate at Asp52
The strained conformation of the D sugar
Formation of a covalent intermediate at Ser195
The abnormally high pKa of Glu35

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Carbohydrate
When living organisms are cooled below some critical body temperature, the metabolic reactions within their cells cease to function properly. This malfunction occurs because

enzyme active sites become permanently bound to substrates
there is insufficient molecular motion for substrates to interact
their enzymes lose the proper three-dimensional shape
the activation energy for the reaction is raised

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