Gel Electrophoresis
In isoelectric focusing, proteins are separated on the basis of their

relative content of positively charged residue only
size
relative content of negatively charged residue only
relative content of positively and negatively charged residue

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Gel Electrophoresis
In an SDS-PAGE

All of these
proteins are denatured by the SDS
smaller proteins migrate more rapidly through the gel
proteins have the same charge-to-mass ratio

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