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Animal Breeding and Transgenic Animal

Animal Breeding and Transgenic Animal
For the transfer of whole individual chromosomes, they are isolated from the cells at

telophase
prophase
metaphase
anaphase

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Animal Breeding and Transgenic Animal
When the cells are enucleated and centrifuged, nuclei

detach from cell and settle at the bottom
None of these
detach from cell and remain in the supernatant
nuclei remain attached to the cell

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Animal Breeding and Transgenic Animal
Enucleation of the cells can be achieved by treating the cells with

both (a) and (b)
alcohol
polyethylene glycol
cytochalasin B

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Animal Breeding and Transgenic Animal
Which of the following is/are the method of transfection for making transgenic animals?

All of these
Transfer of DNA
Transfer of whole individual chromosomes or fragment
Transfer of whole nuclei

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Animal Breeding and Transgenic Animal
Chromosomes may be isolated from metaphase cells by

hypotonic lysis
hypertonic lysis
either (a) or (b)
isotonic lysis

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Animal Breeding and Transgenic Animal
DNA is microinfected into the fertilized egg

any time, it can be infected
at the time of fusion of male and female nuclei
before the fusion of male and female nuclei
after the fusion of male and female nuclei

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