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Polymerase Chain Reaction in Biochemistry

Polymerase Chain Reaction in Biochemistry
DNA fragments in a restriction digest can be separated by electrophoresis in

agarose gel
poly acrylamide
none of these
both (a) and (b)

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Polymerase Chain Reaction in Biochemistry
The PCR, polymerase chain reaction is becoming the method of choice for

sterilization of gene
screening gene
Alteration of gene
all of these

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Polymerase Chain Reaction in Biochemistry
For gene transfer to be effective, transforming DNA must be

free in the bacterial cytoplasm
incorporated into a viral genome
incorporated into the bacterial chromosome
None of these

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Polymerase Chain Reaction in Biochemistry
How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?

Two ds DNA molecule
Eight ds DNA molecule
Three ds DNA molecule
Four ds DNA molecule

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Polymerase Chain Reaction in Biochemistry
PCR can be used to amplify a specific fragment of DNA from which of the following?

All of these
A hair follicle
A drop of blood
A fragment of skin

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Polymerase Chain Reaction in Biochemistry
Double stranded DNA denaturation with specified limit of temperature is

none of these
irreversible reaction
either (a) or (b)
reversible reaction

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