HPLC
An isocratic elution in HPLC is one in which the composition of the solvent

changes continuously
none of these
changes in a series of steps
remains constant

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HPLC
HPLC methods include

liquid/solid (adsorption) chromatography
liquid/liquid (partition) chromatography
ion exchange and size exclusion chromatography
All of these

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HPLC
Void volume refers to the

the time required for the gradient to reach the column
the volume of solvent contained in a liquid chromatographic column
the volume of the column between the point at which solvents are mixed and the beginning of the column
total volume of eluent in the column the remainder being taken up by the packing material

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