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DNA Structure and Replication

DNA Structure and Replication
An enzyme that recognizes foreign DNA, and results in a cut in that DNA is called

restriction endonuclease
helicase
reverse transcriptase
transposase

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DNA Structure and Replication
DNA gyrase in E. coli

is required only at the oriC site
can be inhibited with antibiotics
adds positive supercoils to chromosomal DNA
performs the same function as helicase in eukaryotes

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DNA Structure and Replication
There is a single origin for DNA replication in bacteria. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?

Thousands and tens of thousands
One
none of these
About 100

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DNA Structure and Replication
Which of the following is an active site on its single polypeptide chain for DNA Polymerase I?

All of these
5' → 3' exonuclease
3' → 5' exonuclease
5' → 3' polymerase

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

low temperature and Mg++
low temperature and Ca++
high temperature and Ca++
high temperature and Mg++

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DNA Structure and Replication
In an agarose gel, DNA is moving from

left to right
A to B
cathode to anode
anode to cathode

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