Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

1000 kb and l0kb DNA
10 kb and 1000 kb DNA
2000 kb and 20kb DNA
20 kb and 2000 kb DNA

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would not develop when the substrate was added
The ELISA would develop normally

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