DNA Sequencing, Mutation and Repair
A nonsense mutation introduced into the DNA sequence of a gene may

shorten the length of the protein encoded by the gene
cause a shift in reading frame
cause premature termination of the mRNA
have no effect on the transcript or protein made

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

sequencing the bacterial chromosome while it is still intact
rapidly sequencing thousands of small randomly cloned fragments
methodical sequencing a few large cloned fragments of DNA
All of these

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DNA Sequencing, Mutation and Repair
The DNA sequences of rRNA genes can be used to

predict whether a bacteria will be gram-positive or gram-negative
predict the pulsed field gel pattern of a microorganism
assess relatedness of eukaryotes but not prokaryotes
obtain a tentative identification of a new microorganism

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